- The border between the United States and Florida: The United States claimed that the border should be at the 31st parallel north, while Spain claimed that it should be at the 32nd parallel north.
- The status of slaves who escaped from the United States into Florida: The United States demanded that Spain return escaped slaves, but Spain refused to do so.
- The presence of Native American tribes in Florida who were hostile to the United States: The United States wanted Spain to remove these tribes from Florida, but Spain was unable or unwilling to do so.
- The use of Florida as a base for attacks against the United States by foreign powers: The United States was concerned that Spain was allowing France and Great Britain to use Florida as a base for attacks against the United States.
These disagreements eventually led to the First Seminole War (1817-1818) and the Second Seminole War (1835-1842). The United States eventually acquired Florida from Spain in 1819 as part of the Adams-Onís Treaty.