- Interstate 4 (I-4): Runs east-west across the state, connecting Tampa, Orlando, Daytona Beach, and Jacksonville.
- Interstate 75 (I-75): Runs north-south through the state, connecting Miami, Fort Lauderdale, Orlando, Gainesville, and Lake City.
- Interstate 95 (I-95): Runs along the east coast of the state, connecting Jacksonville, Daytona Beach, Melbourne, West Palm Beach, and Miami.
- Florida's Turnpike (SR 821): A toll road that runs north-south through the state, paralleling I-95.
- State Road A1A (SR A1A): A coastal highway that runs along the Atlantic Ocean, connecting Jacksonville, Daytona Beach, Melbourne, West Palm Beach, and Miami.
- Alligator Alley (I-75): A stretch of I-75 that runs through the Everglades and connects Naples and Fort Lauderdale.
- Overseas Highway (US 1): A scenic highway that connects the Florida Keys to the mainland.