During the Spanish and Mexican mission periods (1791–1834), Mission Santa Cruz operated as a self-sustaining agricultural enterprise. The primary livestock raised included:
These animals were central to the mission economy. Cattle hides and tallow (rendered fat) were the primary export goods in the "hide and tallow trade" with Boston and British ships. The vast mission ranchos (land grants) extended far beyond the mission quadrangle to support these herds. Indigenous neophytes (converts) provided the labor as vaqueros (cowboys), shepherds, and artisans.