Portugal reacted to Spain's claims by challenging the validity of the Treaty of Tordesillas. Portugal argued that the treaty had been signed under false pretenses, and that it was therefore not binding. Portugal also argued that it had already established a presence in the Americas before Spain, and that it therefore had a greater right to the land.
The dispute between Spain and Portugal over the lands of the Americas was eventually resolved in 1529 by the Treaty of Saragossa. This treaty confirmed Spain's control over most of the Americas, while giving Portugal control over Brazil.