- Rivalry and Territorial Claims: Spain had substantial territorial claims in the Americas based on earlier explorations, and Florida fell within the Spanish sphere of influence. They viewed French presence in the region as an infringement on their claims and a threat to Spanish control of the Caribbean and the Gulf of Mexico.
- Trade and Commerce: Florida's strategic location played a crucial role in trade and commerce in the region. Spain wanted to maintain control of these trade routes and markets, which they believed would be disrupted by French competition. Spain feared the competition with the French over Native American trade networks, as well as French attempts to establish trade relationships with Native American groups in the region.
- Religious Differences: Spain was a staunchly Catholic nation and saw the French Protestants, known as Huguenots, as a threat to their religious dominance in the region. Spain wanted to maintain control of Florida to prevent the spread of Protestantism and protect the Catholic faith in the Americas.
- History of Conflict: Spain and France had a long history of conflict and rivalry, both in Europe and in the Americas. The French had previously engaged in privateering activities against Spanish ships, which had led to animosity and distrust. Spain viewed French colonization of Florida as a potential source of future conflicts and tensions.