Yes, the Chumash people staged a significant uprising at Mission San Luis Obispo de Tolosa in 1782. The revolt was fueled by mounting grievances against the mission system: forced labor, harsh discipline, cultural suppression, and devastating European diseases that decimated their communities.
The rebellion was led by a Chumash chief named Tulipay (also spelled Tulipay or Tulipai). Warriors attacked the mission, set fire to buildings, and drove off livestock. Spanish soldiers from the Presidio of Santa Barbara eventually suppressed the revolt, but not before it stood out as one of the largest and most organized indigenous resistance actions in Alta California.
The event underscores the profound disruption the mission system caused to Chumash society, which had thrived for millennia along the Central Coast before Spanish colonization.