The Mississippi River forms a significant portion of the eastern border for three Upper Midwest states:
The Mississippi also borders or flows through Illinois, Missouri, Kentucky, Tennessee, Arkansas, Mississippi, and Louisiana—but for those states, it's a western or internal boundary, not an eastern one. The "three states" framing typically refers to the Upper Mississippi's role as a clean, north-south state line.
These borders were established by the Northwest Ordinance (1787) and subsequent territorial acts, using the river as a clear natural demarcation before survey grids reached the region.