Yes. Despite the 1959 plebiscite where 94% of voters supported statehood (on 35% turnout), significant opposition existed:
Opposition also came from segregationist U.S. senators who resisted adding a majority-non-white state. The statehood bill passed only after Alaska (seen as more Democratic) was admitted first, balancing partisan calculus.
Today, Hawaiian sovereignty movements continue to challenge the legitimacy of the 1898 annexation and 1959 statehood vote, citing suppressed Native participation and international law arguments.