1. Take FL-9A N from Jacksonville to I-95 S.
2. Take I-95 S to I-295 S in Jacksonville.
3. Take I-295 S to FL-202 W in Jacksonville.
4. Take FL-202 W to I-10 W in Lake City.
5. Take I-10 W to I-295 S in Perry.
6. Take I-295 S to I-10 W in Live Oak.
7. Take I-10 W to US-49 S in Madison.
8. Take US-49 S to US-82 W in Tallahatchie County.
9. Take US-82 W to US-61 S in Greenville.
10. Take US-61 S to E Pkwy S in Memphis.
Fastest route by distance (648 miles):
1. Take FL-9A N from Jacksonville to I-95 S.
2. Take I-95 S to I-295 S in Jacksonville.
3. Take I-295 S to I-10 E in Jacksonville.
4. Take I-10 E to I-20 E in Live Oak.
5. Take I-20 E to US-49 S in Winona.
6. Take US-49 S to US-82 W in Tallahatchie County.
7. Take US-82 W to US-61 S in Greenville.
8. Take US-61 S to E Pkwy S in Memphis.
