Remove the covers and sheets from the beds in the hotel room. Check the seams and tufts of the mattress and remove the headboard to look for signs of bed bugs in the room. If you find small red bugs or black fecal spots, the room has an infestation. The room may also have a semi-sweet odor from the bed bugs' pheromones, according to the Virginia Department of Health. You can also look for flea feces, small black dirt that turns red when rubbed between your fingers with a small amount of water.
Spray the mattress with an antibacterial disinfectant spray to kill the fleas and bed bugs on it. Concentrate on the seams of the mattress, spraying any bugs you can see directly with the spray. You can also spray behind the headboard to kill the bugs on contact.
Spray the headboard and around the furniture with an insecticide containing pyrethrin, a natural chemical derived from the chrysanthemum flower. This ingredient kills both fleas and bed bugs, and it is a common component in flea-control spray products.
Sprinkle food-grade diatomaceous earth around the bed, over the furniture, behind the headboard and in the crevices of the mattress. Diatomaceous earth kills bed bugs and fleas by piercing their exoskeletons when they come into contact with it, and does not harm humans.
Spray a solution containing cedar essential oil on the mattress, on furniture and around the floor of the hotel room. Mix 20 drops of cedar oil in 1 cup of rubbing alcohol and pour it into an empty spray bottle. Spray the solution all over the furniture, floor and mattress.
